If you are not certain where to find a particular procedure within STATISTICA, consult the Statistical Advisor by selecting Statistical Advisor from the Help menu. The Statistical Advisor lists a set of questions about the nature of the research problem and the type of your data. Click the link for the most appropriate answer, and the next topic will be displayed, which will either list more questions or list suggestions of the statistical procedures that appear most relevant and where they are located in the STATISTICA application.
In susceptible plants (cv. Thatcher, TcLr34), an oxidative response was observed from 4 to 7 dpi [33]. This coincided with completing the pathogen life cycle and was associated with the development of uredinia, as reported by Orczyk et al. [33]. In these lines, accumulation of H2O2 was significantly alleviated 5 dpi by 2-bromoethylamine indicating the predominant role of DAO in the oxidative response of susceptible plants. In these plants, NADPH oxidase and class III peroxidases were also involved in the accumulation of H2O2 [34] (Figure S1 in Supplementary Materials). In moderately resistant lines (TcLr24, TcLr25 and TcLr29), accumulation of H2O2 steadily increased from 1 to 8 dpi [33] and was associated with a necrosis reaction (Figure 4e,f) [33]. However, neither of them was sufficient to fully arrest pathogen growth and uredinia formation. The results indicated participation of both enzymes in the oxidative response; PAO activity prevailed in the response of TcLr24 (Figure 3c), DAO in TcLr25 (Figure 3d) and both enzymes in the response of TcLr29 (Figure 3e); however, only the changes observed in TcLr29 were statistically significant. Additionally, as previously reported by Dmochowska-Boguta et al. [34], the oxidative response in these lines involved type III peroxidases (POX) in TcLr24 and NADPH oxidases (NOX) along with POX in TcLr25 and TcLr29 (Figure S1 in Supplementary Materials).
statsoft statistica 10 keygen 16
Stratum-specific likelihood ratios and predictive values are presented in Table 3. We found that the likelihood ratio for PSA levels between 4 and 10 ng/ml was statistically equivalent to 1, indicating that no significant probability revision occurred with testing. PSA values less than 2 ng/ml or greater than 20 ng/ml produced the largest probability revisions for detecting prostate cancer.
Differences in frequencies were evaluated using descriptive statistics. Associations between categorical variables were assessed using the χ2 test for independence. Overall survival (OS) was calculated from the date of sampling until the date of last follow-up or death. Survival curves were constructed using the Kaplan-Meier method, and the log-rank test was used to determine differences between survival proportions. For all comparisons, P values were 2-sided and a significance level of P
The strain was isolated from 10 g of sediment, which was diluted in 20 µL sterile seawater and left to decant for 15 min to eliminate the excess particulate material. The supernatant was subsequently diluted 1:10 with sterilized seawater, an aliquot of 100 µL was transferred to agar YMG seawater media with streptomycin (200 mg L-1) and spread over the surface using a sterile glass rod. The growth of fungal colonies was evaluated after 5 days of growth at room temperature and pure strains were obtained from these agar plates (Webster & Weber 2007). In order to validate the morphological identification of E. nigrum strain, we amplified the ITS (which include 5.8S gene) and the nuclear ribosomal large subunit rRNA (LSU) regions using ITS-5 (White et al. 1990) and LR6 (Vilgalys & Hester 1990) primers. DNA was extracted from fruit bodies using the E.Z.N.A. fungal DNA MiniKit (Omega-Biotek). PCR reactions were performed using ITS5 (5'-ggaagtaaaagtcgtaacaagg-3') as forward primer and LR06 (5'-cgccagttctgcttacc-3') as reverse primer. Each reaction was conducted in a 15 µl volume containing 30-50 ng of DNA, 1χ of PCR buffer, 2 mM MgCl2, 0.1 µM of each dNTP, 0.5 µM of forward and reverse primers, and 1 U of Taq DNA polymerase. PCR amplification was carried out with an initial denaturation step of 4 min at 94ºC, and then 35 cycles of 30 s at 94ºC, 60 s at 50ºC and 60 s at 72ºC s, followed by a final step of 5 min at 72ºC. PCR product was sequenced in both senses at Macrogen Inc. (Seoul, Korea). Forward and reverse sequences were edited using Genious v5.4 software (Drummond et al. 2011). The sequence of our E. nigrum strain was analyzed with BLAST (Basic Local Alignment Search Tool, database nucleotide collection nr/nt)1 using Megablast to determine the percentage of maximal identity with the sequences of that global database. Specifically, we performed a neighbor-joining distance tree based on ITS and LSU regions to determine the phylogenetic affinity of our isolated. Finally, the sequence obtained in the present study was deposited in GenBank (CODE: KC164754). Liquid cultures of the strain were carried out in 125 ml of YMG medium, with sterile distilled water (pH 4, 7 and 10) and sterile filtered seawater (pH 4, 7 and 10). Both distilled water and seawater mediums were modified with HCl acid and NaOH alkaline. Incubation was carried out at 6 and 25ºC under constant agitation at 120 rpm for 21 days until the glucose was consumed. The cultures were subsequently filtered and the mycelium was washed with sterile distilled water 3 times to remove all traces of the culture medium. The extraction of sterols was performed from 5 g of mycelium to which 25 mL solution methanol-water (1:1) with NaOH granules, resulting in a concentration of 2N. The mixture was heated for 30 min at 100C, filtered and the supernatant extracted 3 times with a solution of diethyl ether-hexane (1:1), and finally concentrated on a rotary agitator (120 rpm) at 40ºC. The first steps in fatty acid extraction were similar to those of sterol extraction. However, after the mixture was heated for 30 min at 100C, it was cooled to room temperature. Subsequently, a solution of HCl 2N was added until pH 2 was obtained. The mixture was then filtered and the supernatant was extracted 3 times with a solution of diethyl ether-hexane (1:1), and finally concentrated on a rotary agitator (120 rpm) at 40ºC. All extracts were stored at 4ºC for subsequent analysis (Stahl & Klug 1996). The analysis of fatty acid and sterols were carried out by GC-Mass spectrometry (GC-MS) and recorded on a Shimadzu GC-17A/MS QP5050AGC_MS system (electron ionization mode 70 eV, source temperature 270C, scanned mass ranged m/z 43-350). The operating conditions were as follows: 30 m HP-5MS (5%-diphenil-95%-dimethylsiloxane) capillary column; 0.25 mm id, thickness: 0.25 mm, helium as the carrier gas, flow rate of 1 mL min-1 and with split ratio of 1:30, temperature was programmed as follows: from 100C (5 min) to 260C at 10C min-1. The identification of the components of the sterol and fatty acid composition was accomplished by matching their mass spectra with those recorded in the NIST 05 (NIST /EPA/NIH MASS 2005 spectral Library). Spectra were considered coincident if the similarity index was higher than 95%. The Shapiro-Wilk test was used for testing normality of the data and Cochran's test for homogeneity of variances. The effect of cultivation conditions; salinity (i.e., freshwater and seawater); pH (i.e., pH 4, pH 6-7 and pH 10) and temperature (i.e., 6-25C), were compared using the parametric test one-way ANOVA in accordance with its respective response variable in fatty acids and sterols. All statistical calculations were performed using the software package STATISTICA version 6.0 (StatSoft Inc. 2005).
The C16 fatty acids in both salinities showed a higher proportion of saturation. In contrast, the proportion of unsaturated molecules C16 was greater at low temperatures in freshwater and the reverse in seawater cultures (F (112.36)= 0.059) (Tukey's a posteriori P 0.05) (Fig. 4). However, differences in the percentages between the fatty acid molecules were not significant; a similar tendency was observed at basic pH between the different culture media and temperature. Fatty acids extracted from mycelium cultured in freshwater at pH 4, contained higher levels of unsaturated C18:1 and C18:2, with proportions of 64 and 77% at high and low temperatures, respectively. This is in contrast to what was observed in seawater medium where proportions of 24% were reached at low temperatures and 27% at high temperatures (Fig. 5). Unlike the previous cultures, the proportion of saturated fatty acid C16 with 66% at high temperature and 60% at low temperature was greater in seawater medium. Additionally, the proportion of molecules was less for C18:2, with percentages of only 2 and 3% at low and high temperatures respectively (Fig. 5). The differences observed in the production of fatty acid cultures with freshwater and seawater mediums to acidic pH, was statistically significant, differences that would be given by the ratio of fatty acids C18:2 (P = 0.012, d.f.= 1 and C16:0, P = 0.0016, d.f = 1).
The purpose of this page is to make users aware of the latest versions andupdates to statistical software that is commonly used at UCLA. A shortlist of free statistical software is provided at the end of this page. Forthe latest updates for those programs, please visit the link provided.
The last tested LPS replacement substance was R-848. R-848 alone caused visible, but not statistically significant tumor growth reduction. Complete recovery was not observed. However, R-848 combined with anchored mannan resulted in significant synergy and partial recovery of treated mice. Regarding the mechanisms of action, we are considering the important role of granulocytes (neutrophils), as their strong infiltration was noticed. In our previous in vitro experiments, we observed a significant cytotoxic effect of neutrophils against the tumor cells opsonized with mannan. These tumor cells were killed by frustrated phagocytosis [5]. In herein present study, we confirmed our previous observations and by using epicatechin, revealed significant participation of oxidative burst in killing mechanisms. 2ff7e9595c
Comments